Composite

Part:BBa_K2557024:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter--PhiC31-RDF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 408
    Illegal BamHI site found at 1319
    Illegal BamHI site found at 1405
    Illegal BamHI site found at 1652
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 486
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 2328
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1011
    Illegal BsaI.rc site found at 1414
    Illegal BsaI.rc site found at 1767
    Illegal BsaI.rc site found at 2409
    Illegal SapI.rc site found at 3011


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of PhiC31-RDF from the literature and synthesized this sequence.

References